Estimating Bacterial Diversity in Scirtothrips Dorsalis (thysanoptera: Thripidae) via next Generation Sequencing.
نویسندگان
چکیده
The last 2 decades have produced a better understanding of insect-microbial associations and yielded some important opportunities for insect control. However, most of our knowledge comes from model systems. Thrips (Thysanoptera: Thripidae) have been understudied despite their global importance as invasive species, plant pests and disease vectors. Using a culture and primer independent next-generation sequencing and metagenomics pipeline, we surveyed the bacteria of the globally important pest, Scirtothrips dorsalis Hood. The most abundant bacterial phyla identified were Actinobacteria and Proteobacteria and the most abundant genera were Propionibacterium, Stenotrophomonas, and Pseudomonas. A total of 189 genera of bacteria were identified. The absence of any vertically transferred symbiont taxa commonly found in insects is consistent with other studies suggesting that thrips primarilly acquire resident microbes from their environment. This does not preclude a possible beneficial/intimate association between S. dorsalis and the dominant taxa identified and future work should determine the nature of these associations.
منابع مشابه
An Overview of Chilli Thrips, Scirtothrips dorsalis (Thysanoptera: Thripidae) Biology, Distribution and Management
متن کامل
گونۀ Scirtothrips dorsalis (Thysanoptera: Thripidae) به عنوان آفت مرکبات در استان فارس
گونه‌های جنس Scirtothrips Shull حشرات ریزی‌اند که چندین گونۀ آن بر روی محصولات مختلف در دنیا به عنوان آفاتی مهم، شناخته شده‌اند. در این مطالعه، برای نخستین بار گونۀHood S. dorsalis از ایران گزارش می‌شود. رنگ بدن این گونه به‌طور کلی روشن است، اما بندهای انتهایی شاخک تیره‌اند. بندهای استرنیت شکم به جز در ناحیۀ جلویی میانی از ردیف‌های موهای ریز پوشیده شده است. جمعیت زیادی از این گونهدر باغ‌های م...
متن کاملRelationships Among Species of Scirtothrips (Thysanoptera: Thripidae, Thripinae) Using Molecular and Morphological Data
Results of analyses of molecular sequence (COI, 28S-D2) and morphology (21 character states) data, both alone and combined, were used to determine relationships between 18 species of Scirtothrips (Thysanoptera: Thripidae, Thripinae). Two species of Neohydatothrips from Panama were used as the outgroup. Five major pest Scirtothrips were included in these analyses: S. aurantii, S. citri, S. dorsa...
متن کاملDevelopment of a Ribosomal DNA ITS2 Marker for the Identification of the Thrips, Scirtothrips dorsalis
The thrips Scirtothrips dorsalis Hood (Thysanoptera: Thripidae) is an invasive pest that poses a significant economical threat to U.S. agriculture and trade. In this study, DNA sequence data and polymerase chain reaction (PCR) were utilized to develop a molecular diagnostic marker for S. dorsalis. The DNA sequence variation from the internal transcribed spacer 2 (ITS2) region of nuclear ribosom...
متن کاملKey for Identification of Adult Female of Scirtothrips dorsalis (Thysanoptera: Thripidae) Based on External Morphology
Thrips are minute insects and belong to the family thripidae of the order Thysanoptera. Thrips are less than two millimeters long, slender like and agile insects. Head of Scirtothrips dorsalis is pale in colour longer than broad, 217.5-237 μm long and 175-194 μm wide across the eyes. Eyes are redish in colour, 75-89 μm long and 63-70 μm wide at the middle and three occili are arranged triangula...
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ورودعنوان ژورنال:
- The Florida entomologist
دوره 97 2 شماره
صفحات -
تاریخ انتشار 2014